PA1-41170

antibody from Invitrogen Antibodies

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41170

Provider

Invitrogen Antibodies

Product name

AGR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGR2 in MCF7 (A), Caco-2 (B) and human stomach lysate (C). Samples were incubated in AGR2 polyclonal antibody (Product # PA1-41170 using a dilution of 1:100. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGR2 in 0.5 mg/mL MCF-7 lysate. Samples were incubated in AGR2 polyclonal antibody (Product # PA1-41170). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AGR2 in formalin-fixed, paraffin-embedded human adenocarcinoma of the colon. Samples were incubated in AGR2 polyclonal antibody (Product # PA1-41170) using a dilution of 2-5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AGR2 in formalin-fixed, paraffin-embedded human adenocarcinoma of the breast. Samples were incubated in AGR2 polyclonal antibody (Product # PA1-41170) using a dilution of 2 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AGR2 in formalin-fixed, paraffin-embedded normal colon tissue. Samples were incubated in AGR2 polyclonal antibody (Product # PA1-41170) using a dilution of 1:100.