Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-23116 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AGR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGR2 in MCF7 (A), Caco-2 (B) and human stomach lysate (C) . Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116 using a dilution of 1:100. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGR2 in 0.5 mg/mL MCF-7 lysate. Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AGR2 in human adenocarcinoma of the colon. Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116) using a dilution of 2-5 µg/mL followed by a peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AGR2 in human adenocarcinoma of the breast. Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116) using a dilution of 2 µg/mL followed by a peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.