PA5-23116

antibody from Invitrogen Antibodies

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23116

Provider

Invitrogen Antibodies

Product name

AGR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGR2 in MCF7 (A), Caco-2 (B) and human stomach lysate (C) . Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116 using a dilution of 1:100. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGR2 in 0.5 mg/mL MCF-7 lysate. Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AGR2 in human adenocarcinoma of the colon. Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116) using a dilution of 2-5 µg/mL followed by a peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AGR2 in human adenocarcinoma of the breast. Samples were incubated in AGR2 polyclonal antibody (Product # PA5-23116) using a dilution of 2 µg/mL followed by a peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.