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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- ELISA [1]
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- Product number
- NB100-75340 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-75340, RRID:AB_1048701
- Product name
- Chicken Polyclonal NHERF-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NHERF-1
- Reactivity
- Human
- Host
- Chicken/Avian
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proprotein convertase 5/6 is critical for embryo implantation in women: regulating receptivity by cleaving EBP50, modulating ezrin binding, and membrane-cytoskeletal interactions.
Heng S, Cervero A, Simon C, Stephens AN, Li Y, Zhang J, Paule S, Rainczuk A, Singh H, Quinonero A, Tapia A, Velasquez L, Salamonsen L, Rombauts LJ, Nie G
Endocrinology 2011 Dec;152(12):5041-52
Endocrinology 2011 Dec;152(12):5041-52
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Placenta protein extract as test antigen. Affinity-purified IgY dilution: 1:500, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SLC9A3R1 )
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NHERF-1 Antibody [NB100-75340] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of antibody. ( anti-SLC9A3R1 )
