PA5-17044

antibody from Invitrogen Antibodies

Targeting: SLC9A3R1 EBP50, NHERF, NHERF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17044

Provider

Invitrogen Antibodies

Product name

EBP50 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

8 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NHERF1 in extracts from MCF-7 and T-47D cells using NHERF1 polyclonal antibody (Product # PA5-17044).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # PA5-17044) and a 50 kDa band corresponding to EBP50 was observed along with uncharacterized band across cell lines tested. Membrane enriched extracts (30 µg lysate) of Raji (Lane 1), MCF-7 (Lane 2), T-47D (Lane 3), SK-BR-3 (Lane 4), MDA-MB-231 (Lane 5), HeLa (Lane 6), Hep G2 (Lane 7), HEK-293 (Lane 8) and PC-12 (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).