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- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29466 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DMTF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG, mouse testis. Predicted reactivity: Mouse (95%), Chicken (88%), Rhesus Monkey (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.
miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1.
Liu Y, Wang Y, Yao D, Chen X, Zhang F, Feng Y, Li X
Genes 2022 Jan 12;13(1)
Genes 2022 Jan 12;13(1)
miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1.
Yang X, Lou Y, Wang M, Liu C, Liu Y, Huang W
Molecular medicine reports 2019 Mar;19(3):1481-1490
Molecular medicine reports 2019 Mar;19(3):1481-1490
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DMTF1 Polyclonal Antibody (Product # PA5-29466). Sample (50 µg of whole cell lysate). Lane A: mouse testis . 7.5% SDS PAGE. DMTF1 Polyclonal Antibody (Product # PA5-29466) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DMTF1 Polyclonal Antibody (Product # PA5-29466). Sample (30 µg of whole cell lysate). Lane A: PC-3. Lane B: U87-MG . 7.5% SDS PAGE. DMTF1 Polyclonal Antibody (Product # PA5-29466) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. DMTF1 negatively contributes to cell growth. (A) Immunohistochemistry (magnification, x200) and (B) western blot analysis of DMTF1 protein and (C) mRNA levels were examined in human CRC tissue and adjacent normal tissue. (D) Lipofectamine-mediated overexpression of DMTF1 in SW480 was evaluated by reverse transcription-quantitative polymerase chain reaction analysis. In the DMTF1-overexpressing SW480 cells, decreased (E) cell proliferation, (F) single colony formation, (G) colony numbers, and the (H) S phase proportion of the cell cycle were observed. ***P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. DMTF1 restores normal cell growth in miR-675-3p-overexpressing SW480 cells. (A) mRNA levels of DMTF1 were significantly reduced upon introduction of miR-675-3p, but restored by transfection of the DMTF1-expressing plasmid. (B) Protein levels were measured by western blotting in the SW480 cells transfected with miR-NC, miR-675-3p and miR-675-3p + DMTF1, respectively. (C) DMTF1 lowered the miR675-3p-induced elevation in SW480 single colony formation, (D) viable cell proliferation, (E) and the S phase proportion of the cell cycle. ***P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. DMTF1 restores normal cell growth in miR-675-3p-overexpressed HT29 cells. (A) Protein levels were measured by western blotting in HT29 cells transfected with miR-NC, miR-675-3p and miR-675-3p + DMTF1, respectively. (B) DMTF1 lowered the miR675-3p-induced increase in single colony formation of HT29 cells. (C) Cell proliferation was induced by the overexpression of miR-675-3p and restored when DMTF-1 was introduced. ***P
