14414-1-AP

antibody from Invitrogen Antibodies

Targeting: GALE SDR1E1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14414-1-AP

Provider

Invitrogen Antibodies

Product name

GALE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAEKVLVTG GAGYIGSHTV LELLEAGYLP VVIDNFHNAF RGGGSLPESL RRVQELTGRS VEFEEMDILD QGALQRLFKK YSFMAVIHFA GLKAVGESVQ KPLDYYRVNL TGTIQLLEIM KAHGVKNLVF SSSATVYGNP QYLPLDEAHP TGGCTNPYGK SKFFIEEMIR DLCQADKTWN AVLLRYFNPT GAHASGCIGE DPQGIPNNLM PYVSQVAIGR REALNVFGND YDTEDGTGVR DYIHVVDLAK GHIAALRKLK EQCGCRIYNL GTGTGYSVLQ MVQAMEKASG KKIPYKVVAR REGDVAACYA NPSLAQEELG WTAALGLDRM CEDLWRWQKQ NPSGFGTQA (1-348 aa encoded by BC050685 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14414-1-AP (GALE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human malignant melanoma using 14414-1-AP (GALE antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human malignant melanoma using 14414-1-AP (GALE antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GALE (IP:14414-1-AP, 4ug; Detection:14414-1-AP 1:300) with HeLa cells lysate 1080ug.