PA5-22300

antibody from Invitrogen Antibodies

Targeting: ASL ASAL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22300

Provider

Invitrogen Antibodies

Product name

ASL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.

Concentration

0.44 mg/mL

References

Submitted references

l-Citrulline Metabolism in Mice Augments CD4(+) T Cell Proliferation and Cytokine Production In Vitro, and Accumulation in the Mycobacteria-Infected Lung.

Lange SM, McKell MC, Schmidt SM, Hossfeld AP, Chaturvedi V, Kinder JM, McAlees JW, Lewkowich IP, Way SS, Turner J, Qualls JE
Frontiers in immunology 2017;8:1561

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Differential Requirements for L-Citrulline and L-Arginine during Antimycobacterial Macrophage Activity.

Rapovy SM, Zhao J, Bricker RL, Schmidt SM, Setchell KD, Qualls JE
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3293-300

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Argininosuccinate Lyase using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Argininosuccinate Lyase polyclonal antibody (Product # PA5-22300) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASL was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASL Polyclonal Antibody (Product # PA5-22300) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASL Polyclonal Antibody detects ASL protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.10% SDS-PAGE. ASL Polyclonal Antibody (Product # PA5-22300) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells using an Argininosuccinate Lyase polyclonal antibody (Product # PA5-22300) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ASL was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASL Polyclonal Antibody (Product # PA5-22300) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASL Polyclonal Antibody detects ASL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. ASL stained by ASL Polyclonal Antibody (Product # PA5-22300) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ASL (Product # PA5-22300) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASL (Product # PA5-22300) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.