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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
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- Product number
- PA5-22300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12. Predicted reactivity: Mouse (93%), Rat (88%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.78 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references l-Citrulline Metabolism in Mice Augments CD4(+) T Cell Proliferation and Cytokine Production In Vitro, and Accumulation in the Mycobacteria-Infected Lung.
Differential Requirements for L-Citrulline and L-Arginine during Antimycobacterial Macrophage Activity.
Lange SM, McKell MC, Schmidt SM, Hossfeld AP, Chaturvedi V, Kinder JM, McAlees JW, Lewkowich IP, Way SS, Turner J, Qualls JE
Frontiers in immunology 2017;8:1561
Frontiers in immunology 2017;8:1561
Differential Requirements for L-Citrulline and L-Arginine during Antimycobacterial Macrophage Activity.
Rapovy SM, Zhao J, Bricker RL, Schmidt SM, Setchell KD, Qualls JE
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3293-300
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3293-300
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells using an Argininosuccinate Lyase polyclonal antibody (Product # PA5-22300) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ASL was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASL Polyclonal Antibody (Product # PA5-22300) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
