Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-87462 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87462, RRID:AB_11031222
- Product name
- Rabbit Polyclonal Argininosuccinate Lyase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, canine Argininosuccinate Lyase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts.
van Straten G, van Steenbeek FG, Grinwis GC, Favier RP, Kummeling A, van Gils IH, Fieten H, Groot Koerkamp MJ, Holstege FC, Rothuizen J, Spee B
PloS one 2014;9(6):e100077
PloS one 2014;9(6):e100077
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Argininosuccinate Lyase Antibody [NBP1-87462] - Analysis in human cell line HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Argininosuccinate Lyase Antibody [NBP1-87462] - Analysis in human cell lines A-431 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Argininosuccinate Lyase Antibody [NBP1-87462] - Staining of human liver shows moderate nuclear and cytoplasmic positivity in hepatocytes
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Argininosuccinate Lyase Antibody [NBP1-87462] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Argininosuccinate Lyase Antibody [NBP1-87462] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Argininosuccinate Lyase Antibody [NBP1-87462] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Argininosuccinate Lyase Antibody [NBP1-87462] - Staining of human stomach shows weak cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Argininosuccinate Lyase Antibody [NBP1-87462] - Staining in human liver and skeletal muscle tissues using NBP1-87462 antibody. Corresponding ASL RNA-seq data are presented for the same tissues.