Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [2]
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- Product number
- 500-P267BT-25UG - Provider product page

- Provider
- Invitrogen Antibodies
- Product name
- VEGFB Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B. Anti-Human VEGF-B-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF-B (500-P267) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B. Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P267BT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 25 μg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of VEGFB Polyclonal Antibody, Biotin (Product # 500-P267BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech VEGFB Polyclonal Antibody (Product # 500-P267-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of VEGFB Polyclonal Antibody, Biotin (Product # 500-P267BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech VEGFB Polyclonal Antibody (Product # 500-P267-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B.