Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C186115 - Provider product page
- Provider
- LSBio
- Product name
- VIL1 / Villin Antibody (aa7-246) LS-C186115
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. Villin antibody diluted at 1:5000
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Villin antibody detects VIL1 protein by Western blot analysis. A. 50 ug mouse kidney lysate/extract. 7.5 % SDS-PAGE. Villin antibody dilution:1:1000
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Villin antibody detects VIL1 protein by Western blot analysis. A. 50 ug rat kidney lysate/extract. 7.5 % SDS-PAGE. Villin antibody dilution:1:2000
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded A549 xenograft using Villin antibody at 1:500 dilution.