F54302

antibody from NSJ Bioreagents

Targeting: VIL1 D2S1471, VIL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54302

Provider

NSJ Bioreagents

Product name

VIL1 Antibody / Villin 1

Antibody type

Polyclonal

Description

This highly specific VIL1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the VIL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HepG2, 2) human HT-29 and 3) mouse kidney lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) Caco-2 and 2) HT-29 cell lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HT-29, 2) human SW480 and 3) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human kidney tissue with VIL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with VIL1 antibody; Blue=isotype control, Green= VIL1 antibody.