66096-1-IG

antibody from Invitrogen Antibodies

Targeting: VIL1 D2S1471, VIL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66096-1-IG

Provider

Invitrogen Antibodies

Product name

Villin Monoclonal Antibody (2B7B9)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

2B7B9

Vial size

150 µL

Concentration

0.89 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human small intestine tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-IG (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66096-1-IG (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine using 66096-1-Ig (Villin antibody) at dilution of 1:500 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine using 66096-1-Ig (Villin antibody) at dilution of 1:500 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66096-1-Ig (Villin antibody at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66096-1-Ig (Villin antibody at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66096-1-IG ( Villin antibody at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66096-1-IG ( Villin antibody at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Villin (IP:66096-1-IG, 4ug; Detection:66096-1-IG 1:1000) with mouse kidney tissue lysate 6000ug.