Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-17290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Villin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 30 µg/mL
- Storage
- -20°C
Submitted references Establishment of physiologically relevant oxygen gradients in microfluidic organ chips.
Grant J, Lee E, Almeida M, Kim S, LoGrande N, Goyal G, Sesay AM, Breault DT, Prantil-Baun R, Ingber DE
Lab on a chip 2022 Apr 12;22(8):1584-1593
Lab on a chip 2022 Apr 12;22(8):1584-1593
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Villin-1 in extracts from mouse kidney using Villin-1 polyclonal antibody (Product # PA5-17290).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Villin Polyclonal Antibody (Product # PA5-17290) and a 92kDa band corresponding to Villin was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), COLO 205 (Lane 2), Jurkat (Lane 3) and PANC-1 (Lane 4) as seen in Fig (a).Tissue extracts of Mouse Liver (Lane 1), Mouse Colon (Lane 2) and Mouse Jejunum (Lane 3) as seen in Fig (b)were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Villin-1 in HT29 cells using a Villin-1 polyclonal antibody (Product # PA5-17290) (green). DNA is labeled using a fluorescent blue dye.