PA5-90035

antibody from Invitrogen Antibodies

Targeting: PTPRN2 IA-2beta, ICAAR, KIAA0387, phogrin

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90035

Provider

Invitrogen Antibodies

Product name

PTPRN2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AAPSSVPRGR QLPGRLGCLL EEGLCGASEA CVNDGVFGRC QKVPAMDFYR YEVSPVALQR LRVALQKLSG TGFTWQDDYT QYVMDQELAD LPKTYLRRPE ASSPARPSKH SVGSERRYSR EGGAALANAL RRHLPFLEAL SQAPASDVLA RTHTAQDRPP AEGDDRFSES ILTYVAHTSA LTYPPGSRTQ LREDLLPRTL GQLQPDELSP KVDSGVDRHH L; Positive Samples: SH-SY5Y, SGC-7901, Mouse pancreas, Rat pancreas; Cellular Location: Cytoplasmic vesicle, Single-pass type I membrane protein, secretory vesicle, secretory vesicle membrane, synaptic vesicle membrane

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.74 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using PTPRN2 Polyclonal antibody (Product # PA5-90035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PTPRN2 in extracts of various cell lines using PTPRN2 Polyclonal Antibody (Product # PA5-90035) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTPRN2 in paraffin-embedded human mammary cancer using PTPRN2 Polyclonal Antibody (Product # PA5-90035) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTPRN2 in paraffin-embedded human colon carcinoma using PTPRN2 Polyclonal Antibody (Product # PA5-90035) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTPRN2 in paraffin-embedded human lung cancer using PTPRN2 Polyclonal Antibody (Product # PA5-90035) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTPRN2 in paraffin-embedded mouse pancreas using PTPRN2 Polyclonal Antibody (Product # PA5-90035) at a dilution of 1:100.