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LearnNB100-1544
antibody from Novus Biologicals
Targeting: RPS6KB2
KLS, P70-BETA, p70S6Kb, S6KB, S6Kbeta, S6Kβ, STK14B
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-1544 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1544, RRID:AB_10002174
- Product name
- Rabbit Polyclonal p70 S6 Kinase beta/S6K2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cell cycle-dependent nuclear export of phosphatase and tensin homologue tumor suppressor is regulated by the phosphoinositide-3-kinase signaling cascade.
Liu JL, Mao Z, LaFortune TA, Alonso MM, Gallick GE, Fueyo J, Yung WK
Cancer research 2007 Nov 15;67(22):11054-63
Cancer research 2007 Nov 15;67(22):11054-63
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: p70 S6 Kinase beta/S6K2 Antibody [NB100-1544] - Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody NB100-1544 used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody NB100-1545. For blotting immunoprecipitated S6K2, NB100-1545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
