A04055-1
antibody from Boster Biological Technology
Targeting: ASIC2
ACCN, ACCN1, ASIC2a, BNaC1, BNC1, hBNaC1, MDEG
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A04055-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ACCN1/ASIC2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for ACCN1/ASIC2 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Amiloride alleviates morphine tolerance by suppressing ASIC3-dependent neuroinflammation in the spinal cord.
Gei L, Yan Y, Xing W, Li Q, Chen X, Yan F, Wang Y, Cao Y, Jiang W, E R, Luo D, Zhang Y, Zeng W, Chen D
European journal of pharmacology 2024 Jan 15;963:176173
European journal of pharmacology 2024 Jan 15;963:176173
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ASIC2 using anti-ASIC2 antibody (A04055-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat ovarian tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse ovarian tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC2 antigen affinity purified polyclonal antibody (Catalog # A04055-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC2 at approximately 58KD. The expected band size for ASIC2 is at 58KD.
- Additional image