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- Antibody Data
- Antigen structure
- References [22]
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- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF-420-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL11/Eotaxin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human CCL11/Eotaxin is observed, and less than 5% cross-reactivity with recombinant mouse JE is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P48298- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Modulation of the diet and gastrointestinal microbiota normalizes systemic inflammation and β-cell chemokine expression associated with autoimmune diabetes susceptibility.
A role for endothelial cells in radiation-induced inflammation.
CCL11, a novel mediator of inflammatory bone resorption.
Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart.
Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions.
Suppression of laser-induced choroidal neovascularization by a CCR3 antagonist.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
CCR3 is a target for age-related macular degeneration diagnosis and therapy.
IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Sequential ELISA to profile multiple cytokines from small volumes.
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Henschel AM, Cabrera SM, Kaldunski ML, Jia S, Geoffrey R, Roethle MF, Lam V, Chen YG, Wang X, Salzman NH, Hessner MJ
PloS one 2018;13(1):e0190351
PloS one 2018;13(1):e0190351
A role for endothelial cells in radiation-induced inflammation.
Boström M, Kalm M, Eriksson Y, Bull C, Ståhlberg A, Björk-Eriksson T, Hellström Erkenstam N, Blomgren K
International journal of radiation biology 2018 Mar;94(3):259-271
International journal of radiation biology 2018 Mar;94(3):259-271
CCL11, a novel mediator of inflammatory bone resorption.
Kindstedt E, Holm CK, Sulniute R, Martinez-Carrasco I, Lundmark R, Lundberg P
Scientific reports 2017 Jul 13;7(1):5334
Scientific reports 2017 Jul 13;7(1):5334
Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart.
Diny NL, Hou X, Barin JG, Chen G, Talor MV, Schaub J, Russell SD, Klingel K, Rose NR, Čiháková D
European journal of immunology 2016 Dec;46(12):2749-2760
European journal of immunology 2016 Dec;46(12):2749-2760
Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions.
Chao GY, Wallis RH, Marandi L, Ning T, Sarmiento J, Paterson AD, Poussier P
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3645-53
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3645-53
Suppression of laser-induced choroidal neovascularization by a CCR3 antagonist.
Mizutani T, Ashikari M, Tokoro M, Nozaki M, Ogura Y
Investigative ophthalmology & visual science 2013 Feb 28;54(2):1564-72
Investigative ophthalmology & visual science 2013 Feb 28;54(2):1564-72
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Després S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
Nature 2011 Aug 31;477(7362):90-4
Nature 2011 Aug 31;477(7362):90-4
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
The European respiratory journal 2010 Jun;35(6):1377-87
The European respiratory journal 2010 Jun;35(6):1377-87
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
Kearley J, Buckland KF, Mathie SA, Lloyd CM
American journal of respiratory and critical care medicine 2009 May 1;179(9):772-81
American journal of respiratory and critical care medicine 2009 May 1;179(9):772-81
CCR3 is a target for age-related macular degeneration diagnosis and therapy.
Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
Nature 2009 Jul 9;460(7252):225-30
Nature 2009 Jul 9;460(7252):225-30
IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
The Journal of clinical investigation 2007 Oct;117(10):2941-51
The Journal of clinical investigation 2007 Oct;117(10):2941-51
Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.
D'Avila H, Almeida PE, Roque NR, Castro-Faria-Neto HC, Bozza PT
Infection and immunity 2007 Mar;75(3):1507-11
Infection and immunity 2007 Mar;75(3):1507-11
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
Journal of leukocyte biology 2003 Jan;73(1):65-73
Journal of leukocyte biology 2003 Jan;73(1):65-73
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
Journal of leukocyte biology 2003 Jan;73(1):65-73
Journal of leukocyte biology 2003 Jan;73(1):65-73
A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
Reading PC, Symons JA, Smith GL
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1435-42
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1435-42
A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
Reading PC, Symons JA, Smith GL
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1435-42
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1435-42
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
The American journal of pathology 2002 Apr;160(4):1353-60
The American journal of pathology 2002 Apr;160(4):1353-60
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL11/Eotaxin in Mouse Thymus. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL11/Eotaxin and Neutral-ization by Mouse CCL11/ Eotaxin Antibody. Recombinant Mouse CCL11/ Eotaxin (Catalog # 420-ME) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA). The ND50 is typically 0.1-0.5 µg/mL.
