500-P41-1MG

antibody from Invitrogen Antibodies

Targeting: CCL11 eotaxin, MGC22554, SCYA11

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P41-1MG

Provider

Invitrogen Antibodies

Product name

Eotaxin Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Eotaxin (CCL11). Anti-Human Eotaxin (CCL11)-specific antibody was purified by affinity chromatography employing an immobilized Human Eotaxin (CCL11) matrix. Sandwich ELISA: To detect Human Eotaxin (CCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Eotaxin (CCL11) (500-P41Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin (CCL11). Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

1 mg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

TARC and IL-5 expression correlates with tissue eosinophilia in peripheral T-cell lymphomas.

Thielen C, Radermacher V, Trimeche M, Roufosse F, Goldman M, Boniver J, de Leval L
Leukemia research 2008 Sep;32(9):1431-8

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Degranulating eosinophils in human endometriosis.

Blumenthal RD, Samoszuk M, Taylor AP, Brown G, Alisauskas R, Goldenberg DM
The American journal of pathology 2000 May;156(5):1581-8

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Differential chemokine expression in tissues involved by Hodgkin's disease: direct correlation of eotaxin expression and tissue eosinophilia.

Teruya-Feldstein J, Jaffe ES, Burd PR, Kingma DW, Setsuda JE, Tosato G
Blood 1999 Apr 15;93(8):2463-70

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CD26/dipeptidyl-peptidase IV down-regulates the eosinophil chemotactic potency, but not the anti-HIV activity of human eotaxin by affecting its interaction with CC chemokine receptor 3.

Struyf S, Proost P, Schols D, De Clercq E, Opdenakker G, Lenaerts JP, Detheux M, Parmentier M, De Meester I, Scharpé S, Van Damme J
Journal of immunology (Baltimore, Md. : 1950) 1999 Apr 15;162(8):4903-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hEotaxin by Western Blot analysis Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hEotaxin by Western Blot analysis Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human Eotaxin (CCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Eotaxin Polyclonal Antibody, Biotin (Product # 500-P41BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin (CCL11).