MAB272-100

antibody from Novus Biologicals

Targeting: CCL1 I-309, P500, SCYA1, SISe, TCA3

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB272-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal CCL1/I-309/TCA-3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CCL1/I-309/TCA-3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma , 11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma , 11, 21, or 25 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CCL1/I-309 and Neutralization by Human CCL1/I-309 Anti-body. Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I-309 Monoclonal Antibody (Catalog # MAB272). The ND₅₀ is typically 0.1-0.5 µg/mL.