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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB8451 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL1/I-309/TCA-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL1/I-309/TCA-3 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL1, recombinant mouse (rm) CCL17, rmCCL19 or rmCCL24 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P10146- Isotype
- IgG
- Antibody clone number
- 148107
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
The American journal of pathology 2015 Feb;185(2):420-31
The American journal of pathology 2015 Feb;185(2):420-31
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
Kearley J, Robinson DS, Lloyd CM
The Journal of allergy and clinical immunology 2008 Sep;122(3):617-24.e6
The Journal of allergy and clinical immunology 2008 Sep;122(3):617-24.e6
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL1/I-309/TCA-3 and Neutralization by Mouse CCL1/I-309/TCA-3 Antibody. Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL1/I-309/ TCA-3 Monoclonal Antibody (Catalog # MAB8451). The ND50 is typically 0.6-3.0 µg/mL.
