Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB845-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL1/I-309/TCA-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL2, 3, 4,5,6, 7, 8, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P10146
- Isotype
- IgG
- Antibody clone number
- 148113
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
Islam SA, Chang DS, Colvin RA, Byrne MH, McCully ML, Moser B, Lira SA, Charo IF, Luster AD
Nature immunology 2011 Feb;12(2):167-77
Nature immunology 2011 Feb;12(2):167-77
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
Kearley J, Buckland KF, Mathie SA, Lloyd CM
American journal of respiratory and critical care medicine 2009 May 1;179(9):772-81
American journal of respiratory and critical care medicine 2009 May 1;179(9):772-81
Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
Yang L, Mosmann T
European journal of immunology 2004 Jun;34(6):1617-26
European journal of immunology 2004 Jun;34(6):1617-26
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL1/ITCA-3 and Neutralization by Mouse CCL1/ITCA-3 Antibody. Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA-3 Monoclonal Antibody (Catalog # MAB845). The ND50 is typically 2-10 µg/mL.