Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF272 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL1/I-309/TCA-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CCL1/I-309/TCA-3 in direct ELISAs and Western blots. Neutralizes the biological activity of recombinant human CCL1. Does not block the biological activity of recombinant mouse TCA-3.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22362
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Comprehensive assessment of chemokine expression profiles by flow cytometry.
CC chemokine ligand 1 is released into the airways of atopic asthmatics.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
CC chemokine ligand 1 is released into the airways of atopic asthmatics.
Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-García MC, Velásquez JR, Teran LM
The European respiratory journal 2006 Jul;28(1):59-67
The European respiratory journal 2006 Jul;28(1):59-67
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL1/I-309 and Neutralization by Human CCL1/I-309 Antibody. Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL1/I-309 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF272). The ND50 is typically 0.4-2.0 µg/mL.