102-PA25S

antibody from ReliaTech GmbH

Targeting: KRIT1 CAM, CCM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

102-PA25S

Provider

ReliaTech GmbH

Product name

CCM-1

Antibody type

Polyclonal

Antigen

Recombinant human CCM1

Description

antibody Protein-A purified from serum

Reactivity

Human

Host

Rabbit

Antigen sequence

MGSSHHHHHHSSGLVPRGSHMGNPENIEDAYVAVI
RPKNTASLNSREYRAKSYEILLHEVPIEGQKKKRK
KVLLETKLQGNSEITQGILDYVVETTKPISPANQG
IRGKRVVLMKKFPLDGEKMGREASLFIVPSVVKDN
TKYTYTPGCPIFYCLQDIMRVCSESSTHFATLTAR
MLIALDKWLDERHAQSHFIPALFRPSPLERIKTNV
INPAYATESGQTENSLHMGYSALEIKSKMLALEKA
DTCIYNPLFGSDLQYTNRVDKVVINPYFGLGAPDY
SKIQIPKQEKWQRSMSSVTEDKERQWVDDFPLHRS
ACEGDSELLSRLLSERFSVNQLDSDHWAPIHYACW
YGKVEATRILLEKGKCNPNLLNGQLSSPLHFAAGG
GHAEIVQILLNHPETDRHITDQQGRSPLNICEENK
QNNWEEAAKLLKEAINKPYEKVRIYRMDGSYRSVE
LKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENL
SLQLKPYHKPLQHVRDWPEILAELTNLDPQRETPQ
LFLRRDVRLPLEVEKQIEDPLAILILFDEARYNLL
KGFYTAPDAKLITLASLLLQIVYGNYESKKHKQGF
LNEENLKSIVPVTKLKSKAPHWTNRILHEYKNLST
SEGVSKEMHHLQRMFLQNCWEIPTYGAAFFTGQIF
TKASPSNHKVIPVYVGVNIKGLHLLNMETKALLIS
LKYGCFMWQLGDTDTCFQIHSMENKMSFIVHTKQA
GLVVKLLMKLNGQLMPTERNS


Antibody clone number

Rabbit IG

Vial size

100 µl

Storage

Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

Handling

Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Western analysis of recombinant human CCM-1 (FERM domain) and recombinant human full length CCM-1 using a rabbit polyclonal anti-human CCM-1 antibody [Cat# 102-PA25] generated against the recombinant FERM domain of human CCM-1.

Sample type

Purified recombinant proteins

Immunohistochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Immunofluorescence staining of human foreskin (cryo-section of unfixed tissue) with anti-CCM1 (red; dilution 1:50) [Cat# 102-PA25]. Costaining of endothelial cells with anti-CD31 (green). Note specific staining in the wall of a subset of vessel. Nuclei counter-stained with Dapi (blue). Specimen provided by Prof. Dr. J. Wilting and Dr. K. Buttler, Goettingen. The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.

Sample type

Human Foreskin