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Flow cytometry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-23859 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD153 Monoclonal Antibody (116614)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human (rh) CD27 Ligand, recombinant mouse CD30 Ligand, or rhCD40 Ligand is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 116614
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CD153 in Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight. Samples were incubated in either (A) CD153 monoclonal antibody (Product # MA5-23859) or (B) Mouse IgG2B Isotype Control followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CD153 in HDLM human Hodgkin’s lymphoma cell line. Samples were incubated in CD153 monoclonal antibody (Product # MA5-23859) using a dilution of 1 µg/mL. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL), Recombinant Human CD30 Ligand/TNFSF8 stimulates IL‚6 secretion in the HDLM human Hodgkin’s lymphoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‚6 Quantikine ELISA Kit. Under these conditions, IL‚6 secretion elicited by Recombinant Human CD30 Ligand/TNFSF8 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD30 Ligand/TNFSF8 Monoclonal Antibody. The ND50 is typically 1-4 µg/mL.
