PA1-317

antibody from Invitrogen Antibodies

Targeting: CBFB PEBP2B

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-317

Provider

Invitrogen Antibodies

Product name

CBF beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-317 detects CBF-beta in human samples. PA1-317 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates. The PA1-317 immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta. This sequence is conserved in mouse. PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression.

Hsu CH, Ma HP, Ong JR, Hsieh MS, Yadav VK, Yeh CT, Chao TY, Lee WH, Huang WC, Kuo KT, Fong IH, Lin CC, Su CM
Disease markers 2022;2022:8446629

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Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive Binding of Core-Binding Factor Beta.

Miyagi E, Welbourn S, Sukegawa S, Fabryova H, Kao S, Strebel K
Journal of virology 2020 Mar 17;94(7)

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CBFβ enhances de novo protein biosynthesis of its binding partners HIV-1 Vif and RUNX1 and potentiates the Vif-induced degradation of APOBEC3G.

Miyagi E, Kao S, Yedavalli V, Strebel K
Journal of virology 2014 May;88(9):4839-52

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Novel function of the unique N-terminal region of RUNX1c in B cell growth regulation.

Brady G, Elgueta Karstegl C, Farrell PJ
Nucleic acids research 2013 Feb 1;41(3):1555-68

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A stable transcription factor complex nucleated by oligomeric AML1-ETO controls leukaemogenesis.

Sun XJ, Wang Z, Wang L, Jiang Y, Kost N, Soong TD, Chen WY, Tang Z, Nakadai T, Elemento O, Fischle W, Melnick A, Patel DJ, Nimer SD, Roeder RG
Nature 2013 Aug 1;500(7460):93-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of CBF beta from HeLa whole cell lysates using Product # PA1-317.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8. RUNX1c and RUNX1b interact equally with CBF-beta. ( A ) The 10-cm dishes of HEK293T cells were transfected with 8 ug of pCEP4, pCEP-RUNX1c or pCEP-RUNX1b. Cells were lysed the following day and used for CBF-beta-His protein pull-downs. A small sample of each lysate was immunoblotted for the input level of RUNX1, using GAPDH as a loading control. CBF-beta-His levels on the nickel beads were assessed by staining the gel with Coomassie blue. Pull-down material was immunoblotted and probed for RUNX1. ( B ) CBF-beta-His pull-downs in extracts from IB4 stable pMEP4-RUNX1cTT and RUNX1bTT induced with CdCl 2 showing pulled down material and lysate input probed for RUNX1 expression. ( C ) HEK293T cells were transfected with pBKCMV-CBF-beta-flag alone or with pCEP-RUNX1c or pCEP-RUNX1b. Cells were lysed the following day. Anti-flag pull-downs were performed, and the material was immunoblotted and probed for RUNX1 and CBF-beta-flag. ( D ) HEK293T cells were transfected with indicated plasmids, lysed the following day and immunoblotted for RUNX1 (blot re-probed with a BZLF1 antibody), FLAG and GAPDH. ( E ) IB4 pMEP4 stable cell lines expressing TT alone, RUNX1cTT or RUNX1bTT induced with 1 uM of CdCl 2 for 24 h. Extracts were immunoblotted to determine the effect on endogenous CBF-beta levels. Data are representative of three separate experiments.