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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A500-003A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Menin Monoclonal Antibody (2G3G8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G3G8
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Menin by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Menin monoclonal antibody [2G3G8] (Product # A500-003A lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Menin by WB and IP of HeLa cells. Antibodies: Mouse monoclonal anti-Menin [2G3G8] (A500-003A) and rabbit anti-Menin antibody (A300-105A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Menin in FFPE prostate carcinoma by IHC. Antibody: Mouse monoclonal anti-Menin antibody [2G3G8] (A500-003A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Menin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Menin monoclonal antibody [2G3G8] (Product # A500-003A lot 1) used for IP at 3 µL per reaction. Menin was also immunoprecipitated by rabbit anti-Menin antibody (Product # A300-105A). For blotting immunoprecipitated Menin, A500-003A was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
