Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB2814 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GLP-1R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human GLP-1R transfectants but not irrelevant transfectants in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_002053
- Isotype
- IgG
- Antibody clone number
- 197920
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Molecular Characterisation of Small Molecule Agonists Effect on the Human Glucagon Like Peptide-1 Receptor Internalisation.
Thompson A, Stephens JW, Bain SC, Kanamarlapudi V
PloS one 2016;11(4):e0154229
PloS one 2016;11(4):e0154229
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GLP-1R in HEK293 Human Cell Line Transfected with Human GLP-1R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GLP-1R and eGFP was stained with and either (A) Mouse Anti-Human GLP-1R Monoclonal Antibody (Catalog # MAB2814) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.