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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22316 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM32 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, DDDDK-TRIM32-transfected 293T.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIM32 Polyclonal Antibody detects TRIM32 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract.7.5% SDS-PAGE. TRIM32 Polyclonal Antibody (Product # PA5-22316) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIM32 Polyclonal Antibody detects TRIM32 protein by western blot analysis. A. 5 µg 293T whole cell lysate/extract. B. 5 µg whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells.7.5% SDS-PAGE. TRIM32 Polyclonal Antibody (Product # PA5-22316) dilution: 1:20,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIM32 Polyclonal Antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 Polyclonal Antibody (Product # PA5-22316) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TRIM32 was performed in paraffin-embedded mouse brain tissue using TRIM32 Polyclonal Antibody (Product # PA5-22316) at a dilution of 1:400.
