PA5-120841

antibody from Invitrogen Antibodies

Targeting: EIF4G2 DAP5, NAT1, p97

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120841

Provider

Invitrogen Antibodies

Product name

DAP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HeLa, 293T, Jurkat, LO2, Mouse brain. Immunogen sequence: PKTINQIRQD AVKDLGVFIP APMAQGMRSD FFLEGPFMPP RMKMDRDPLG GLADMFGQMP GSGIGTGPGV IQDRFSPTMG RHRSNQLFNG HGGHIMPPTQ SQFGEMGGKF MKSQGLSQLY HNQSQGLLSQ LQGQSKDMPP RFSKKGQLNA DEISLRPAQS FLMNKNQVPK L

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.26 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DAP5 in C6 cells. Samples were incubated with DAP5 Polyclonal antibody (Product # PA5-120841) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DAP5 in HeLa cells. Samples were incubated with DAP5 Polyclonal antibody (Product # PA5-120841) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DAP5 in NIH-3T3 cells. Samples were incubated with DAP5 Polyclonal antibody (Product # PA5-120841) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of DAP5 in 300 μg extracts of Hela cells. Samples were precipitated with 3 μg DAP5 Polyclonal antibody (Product # PA5-120841). Western blot was performed from the immunoprecipitate using DAP5 Polyclonal antibody (Product # PA5-120841) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DAP5 in paraffin-embedded mouse brain. Samples were incubated with DAP5 Polyclonal antibody (Product # PA5-120841) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.