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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB120-11929 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-11929, RRID:AB_921104
- Product name
- Mouse Monoclonal DAP5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In Jurkat, a 97 kDa band is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The mammalian host protein DAP5 facilitates the initial round of translation of Coxsackievirus B3 RNA.
Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle.
Dave P, George B, Raheja H, Rani P, Behera P, Das S
The Journal of biological chemistry 2019 Oct 18;294(42):15386-15394
The Journal of biological chemistry 2019 Oct 18;294(42):15386-15394
Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle.
Escobar J, Frank JW, Suryawan A, Nguyen HV, Davis TA
American journal of physiology. Endocrinology and metabolism 2007 Dec;293(6):E1615-21
American journal of physiology. Endocrinology and metabolism 2007 Dec;293(6):E1615-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAP5 Antibody (39C534.1) [NB120-11929] - Analysis of NB120-11929 (1ug) on 15 ug of total cell lysate from Jurkat cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DAP5 Antibody (39C534.1) [NB120-11929] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
