H00002821-M01

antibody from Abnova Corporation

Targeting: GPI AMF, NLK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002821-M01

Provider

Abnova Corporation

Product name

GPI monoclonal antibody (M01), clone 8B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant GPI.

Antigen sequence

MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKD
RFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVD
LAKSRGVEAARERMFNGEKINYTEGRAVLHVALRN
RSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGD
WKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYS
SGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIAS
KTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVA
LSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAI
GLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKN
APVLLALLGIWYINCFGCETHAMLPYDQYLHRFAA
YFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGT
NGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGL
HHKILLANFLAQTEALMRGKSTEEARKELQAAGKS
PEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGAL
VAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKI
EPELDGSAQVTSHDASTNGLINFIKQQREARVQ


Isotype

IgG

Antibody clone number

8B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GPI monoclonal antibody (M01), clone 8B8. Western Blot analysis of GPI expression in MCF-7.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GPI expression in transfected 293T cell line by GPI monoclonal antibody (M01), clone 8B8.Lane 1: GPI transfected lysate (Predicted MW: 63.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GPI is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol