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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34724 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPI Recombinant Rabbit Monoclonal Antibody (JG33-86)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, HepG2, LO2, MCF-7, human colon tissue, human prostate tissue, human kidney tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG33-86
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glucose 6 phosphate isomerase in A549 and HepG2 cells lysates. Samples were incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glucose 6 phosphate isomerase in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glucose 6 phosphate isomerase in LO2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glucose 6 phosphate isomerase in paraffin-embedded human prostate tissue. Samples were incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glucose 6 phosphate isomerase in paraffin-embedded human kidney tissue. Samples were incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glucose 6 phosphate isomerase in paraffin-embedded human colon tissue. Samples were incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Glucose 6 phosphate isomerase in A549 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Glucose 6 phosphate isomerase monoclonal antibody (Product # MA5-34724) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
