Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-19224 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with porcine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Heart lysate using Product # PA5-19224 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPI by a GPI monoclonal antibody (Product # PA5-19224) at a concentration of 0.05 µg/mL. HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GPI in Human Thyroid using a GPI monoclonal antibody (Product #PA5-19224) at 3.75 µg/mL. The Human Thyroid tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GPI in Human Testis using a GPI monoclonal antibody (Product #PA5-19224) at 3.75 µg/mL. The Human Testis tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GPI in Human Thyroid using a GPI monoclonal antibody (Product #PA5-19224) at 3.75 µg/mL. The Human Thyroid tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GPI in Human Kidney using a GPI monoclonal antibody (Product #PA5-19224) at 3.75 µg/mL. The Human Kidney tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.