Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
- Other assay [3]
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Validation data
Reference
Comment
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- Product number
- PA5-26787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Type I interferon decreases macrophage energy metabolism during mycobacterial infection.
Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.
Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease.
Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation.
Olson GS, Murray TA, Jahn AN, Mai D, Diercks AH, Gold ES, Aderem A
Cell reports 2021 Jun 1;35(9):109195
Cell reports 2021 Jun 1;35(9):109195
Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.
Wu L, Hollinshead KER, Hao Y, Au C, Kroehling L, Ng C, Lin WY, Li D, Silva HM, Shin J, Lafaille JJ, Possemato R, Pacold ME, Papagiannakopoulos T, Kimmelman AC, Satija R, Littman DR
Cell 2020 Aug 6;182(3):641-654.e20
Cell 2020 Aug 6;182(3):641-654.e20
Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease.
Clotet S, Soler MJ, Riera M, Pascual J, Fang F, Zhou J, Batruch I, Vasiliou SK, Dimitromanolakis A, Barrios C, Diamandis EP, Scholey JW, Konvalinka A
Molecular & cellular proteomics : MCP 2017 Mar;16(3):368-385
Molecular & cellular proteomics : MCP 2017 Mar;16(3):368-385
Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation.
Araujo L, Khim P, Mkhikian H, Mortales CL, Demetriou M
eLife 2017 Jan 6;6
eLife 2017 Jan 6;6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a GPI polyclonal antibody (Product # PA5-26787) in mouse Neuro-2a cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a GPI polyclonal antibody (Product # PA5-26787) in A2058 (lane 1), Y79 (lane 2), Ramos (lane 3), A375 (lane 4), K562 (lane 5) and mouse Neuro-2a (lane 6) cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Ramos cells using a GPI polyclonal antibody (Product # PA5-26787) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. N-glycan branching controls T H 17 versus iTreg cell fate. ( A ) Fructose 6-phosphate and glutamine may be metabolized by glycolysis and glutaminolysis, respectively, or enter the hexosamine pathway to supply UDP-GlcNAc to the Golgi branching enzymes Mgat1, 2, 4 and 5. HK: hexokinase, GPI: glucose-6-phosphate isomerase, PFK1: phosphofructokinase1, LDH: Lactate dehydrogenase, GFPT: glutamine-fructose-6-phosphate transaminase. ( B - H ) Flow cytometry ( B , E - H ), Western blot ( C ) and LC-MS/MS ( D ) analysis of purified mouse splenic CD4 + T-cells activated with anti-CD3+anti-CD28 for 4 days ( B , E - H ) or 3 days ( C,D ) with T H 17 inducing conditions (TGFbeta+IL-6+IL-23) or as indicated. PFK1-L (liver), PFK1-P (platelet), PFK1-M (muscle). ( G ) Co-incubation with doxycycline in vitro. ( H ) Doxycycline treatment in vivo, with Mgat1 f/f tetO-Cre + ROSA rtTA cells in right panel gated on L-PHA - population. ( B , E - H ) gated on CD4 + . ( B , D - H ) Unpaired two tailed t -test with Welch's ( E ) and Bonferroni corrections ( B , E ). **p