24641-1-AP

antibody from Proteintech Group

Targeting: TOP2A TOP2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

24641-1-AP

Provider

Proteintech Group

Product name

TOP2A antibody

Antibody type

Polyclonal

Description

KD/KO validated TOP2A antibody (Cat. #24641-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Dexrazoxane Protects Against Hand-Foot Syndrome-Like Skin Damage in Pegylated Liposomal Doxorubicin-Treated Mice.

Nishida K, Tanaka J, Hashimoto C, Tanaka M, Kuga T, Shigeta S, Kitatani K
Journal of toxicology 2026;2026:1358796

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STUB1 downregulates TOP2A through a dual mechanism of ubiquitination and FOXM1-mediated transcription repression, suppressing breast cancer growth and enhancing sensitivity to chemotherapy.

Yue B, Xiang Q, Qiu H, Huang M, Qi M, Yi X, Zhou S, Xiong J
Cellular & molecular biology letters 2026 Mar 18;31(1)

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PBX3 regulates mast cell parthanatos via TOP2A mediated DNA damage in allergic rhinitis.

Wu S, Zhu X, Xie W, Liu J, Li C, Wang M, Peng H
iScience 2026 Apr 17;29(4):115426

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Multi-omics reveals the polyethylene terephthalate carcinogenicity: Cancer progression and immune microenvironment.

Yang K, Zhou X, Wu K, Wu J, Huang C, Yang L
Ecotoxicology and environmental safety 2025 Sep 1;302:118522

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Novel β-carboline derivatives show promise as dual-target inhibitors of DNA and TOP2A for the treatment of triple negative breast cancer.

Xu X, Lu X, Liu X, Ling Y, Yang Y, Jiang A
European journal of medicinal chemistry 2025 Nov 15;298:118041

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TOP2A Promotes Proliferation, Migration, and Inflammatory Response in M5-Treated Keratinocytes by Binding CTBP1 to Activate Wnt/β-Catenin Signaling.

Yang Y, Liu S, Xiao X
Cell biochemistry and biophysics 2025 Jun;83(2):2101-2113

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A single-cell transcriptomic atlas of human lens epithelium: identification and functional insights into lens stem/progenitor cells.

Gu Y, Chen L, Chen S, Wu Y, Hao S, Sheng F, Yuan J, Qin Z, Wu D, Han Y, Yao Z, Chen Z, Wormstone IM, Yu Y, Qian J, Fu Q, Yao K
Stem cell research & therapy 2025 Jul 1;16(1):333

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Comparative proteomic landscapes elucidate human preimplantation development and failure.

Zhu W, Meng J, Li Y, Gu L, Liu W, Li Z, Shen Y, Shen X, Wang Z, Wu Y, Wang G, Zhang J, Zhang H, Yang H, Dong X, Wang H, Huang X, Sun Y, Li C, Mu L, Liu Z
Cell 2025 Feb 6;188(3):814-831.e21

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Identification of ubiquitin markers for survival and prognosis of ovarian cancer.

Feng Y, Shan L, Gong Y, Hang W, Sang Z, Sun Y, Tang K, Wang Y, Hu B, Xi X
Heliyon 2024 Sep 30;10(18):e37288

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Expression and biological significance of topoisomerase II α (TOP2A) in oral squamous cell carcinoma.

Cheng X, Wei Y, Deng L, Dong H, Wei H, Xie C, Tuo Y, Chen M, Qin H, Cao Y
Discover oncology 2024 Sep 10;15(1):423

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Transcriptomic profiling highlights cell proliferation in the progression of experimental pulmonary hypertension in rats.

Luo A, Hao R, Zhou X, Jia Y, Bao C, Yang L, Zhou L, Gu C, Desai AA, Tang H, Chu AA
Scientific reports 2024 Jun 18;14(1):14056

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Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer.

Ji T, Fu H, Wang L, Chen J, Tian S, Wang G, Wang L, Wang Z
PLoS genetics 2024 Feb;20(2):e1011176

---

FN1 mediated activation of aspartate metabolism promotes the progression of triple-negative and luminal a breast cancer.

Chen C, Ye L, Yi J, Liu T, Li Z
Breast cancer research and treatment 2023 Oct;201(3):515-533

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O-GlcNAcylation promotes topoisomerase IIα catalytic activity in breast cancer chemoresistance.

Liu Y, Yu K, Zhang K, Niu M, Chen Q, Liu Y, Wang L, Zhang N, Li W, Zhong X, Li G, Wu S, Zhang J, Liu Y
EMBO reports 2023 Jul 5;24(7):e56458

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Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation.

Muneer A, Wang L, Xie L, Zhang F, Wu B, Mei L, Lenarcic EM, Feng EH, Song J, Xiong Y, Yu X, Wang C, Jain K, Strahl BD, Cook JG, Wan YY, Moorman NJ, Song H, Jin J, Chen X
Cell chemical biology 2023 Dec 21;30(12):1525-1541.e7

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Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC.

Chen MY, Zeng YC, Zhao XH
Frontiers in cell and developmental biology 2022;10:893490

---

Kinase activity profiling identifies putative downstream targets of cGMP/PKG signaling in inherited retinal neurodegeneration.

Roy A, Tolone A, Hilhorst R, Groten J, Tomar T, Paquet-Durand F
Cell death discovery 2022 Mar 3;8(1):93

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High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas.

Zhou X, Niu X, Mao Q, Liu Y
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2021 May;87:59-65

---

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

Wang L, Muneer A, Xie L, Zhang F, Wu B, Mei L, Lenarcic EM, Feng EH, Song J, Xiong Y, Yu X, Wang C, Gheorghe C, Torralba K, Cook JG, Wan YY, Moorman NJ, Song H, Jin J, Chen X
bioRxiv : the preprint server for biology 2020 Nov 16;

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SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.

Watanabe R, Maekawa M, Hieda M, Taguchi T, Miura N, Kikugawa T, Saika T, Higashiyama S
Molecular biology of the cell 2020 Mar 15;31(6):478-490

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Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.

Martin JH, Nixon B, Lord T, Bromfield EG, Aitken RJ
Developmental biology 2016 Sep 1;417(1):63-76

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SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Wu J, Yang C, Guo C, Li X, Yang N, Zhao L, Hang H, Liu S, Chu P, Sun Z, Sun B, Lin Y, Peng J, Han G, Wang S, Tang Z
Chemico-biological interactions 2016 Jan 25;244:94-104

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Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: a proteomic study.

Liu JS, He SC, Zhang ZL, Chen R, Fan L, Qiu GL, Chang S, Li L, Che XM
Oncology reports 2014 Dec;32(6):2635-47

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP( TOP2A Antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The TOP2A antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human TOP2A. This antibody recognizes human antigen. The TOP2A antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.

Appendix

24641-1-AP.pdf

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