20233-1-AP

antibody from Proteintech Group

Targeting: TOP2A TOP2

Provider product page for 20233-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [29]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20233-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20233-1-AP, RRID:AB_10664923
Product name: TOP2A-Specific antibody
Antibody type: Polyclonal
Description: KD/KO validated TOP2A-Specific antibody (Cat. #20233-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TOP2A protein structure - 20233-1-AP shown in red.

Submitted references The SMC5/SMC6 complex is critical for resolving R-loop-induced transcription-replication conflicts.

Wu T, Li Y, Zhao Y, Bournique E, Ortega P, Nie M, Wang Y, Wang H, Buisson R, Hickson ID, Boddy MN, Wu X
Nucleic acids research 2026 Jan 14;54(2)

Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System.

Tomioka S, Kitazawa R, Haraguchi R, Takaoka Y, Inoue A, Kitazawa S
Acta histochemica et cytochemica 2026 Feb 27;59(1):1-11

A four-gene signature identified by integrated transcriptomic analysis for differential diagnosis and prognosis of uterine smooth muscle tumors.

Hu H, Chen Y, Hong B, Liu J, Jiang Y, Yu Z, Xiao ZJ, Li J
Frontiers in oncology 2025;15:1591875

Prevention of Heart Failure Induced by Doxorubicin with Early Administration of Dexrazoxane (PHOENIX Study): dose response and time course of dexrazoxane-induced degradation of topoisomerase 2b.

Chang HM, Hsu JY, Ahn C, Yeh ETH
Cardio-oncology (London, England) 2025 May 2;11(1):42

MYBL2 promotes proliferation of clear cell renal cell carcinoma by regulating TOP2A and activating AKT/mTOR signaling pathway.

Huang Y, Xi X, Ye Z, Zhang C, Jiang Y, Yu F, Huang G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jan 31;39(2):e70330

Topoisomerase IIα orchestrates secretion of IL-6 and IL-8 with human papillomavirus replication.

Liu Y, Han Z, Kaminski P, Tao C, Li X, Liu M, Li Y, Jia Y, Xu J, Hong S
Virologica Sinica 2025 Dec;40(6):946-961

Groenlandicine enhances cisplatin sensitivity in cisplatin-resistant osteosarcoma cells through the BAX/Bcl-2/Caspase-9/Caspase-3 pathway.

Zhao Z, Wu Q, Xu Y, Qin Y, Pan R, Meng Q, Li S
Journal of bone oncology 2024 Oct;48:100631

Single-cell RNA sequencing reveals the intercellular crosstalk and the regulatory landscape of stromal cells during the whole life of the mouse ovary.

Jiang W, Sun W, Peng Y, Xu H, Fan H, Jin X, Xiao Y, Wang Y, Yang P, Shu W, Li J
Life medicine 2024 Dec;3(6):lnae041

TuBG1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways.

Zhang Y, Wang ZZ, Han AQ, Yang MY, Zhu LX, Pan FM, Wang Y
Hepatobiliary & pancreatic diseases international : HBPD INT 2024 Apr;23(2):195-209

Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation.

Song C, Chen H, Yu B, Zhang L, Wang J, Feng C, Yang X, Tian X, Fan Y, Ji X, Wang H, Xie C, Zhang X
Research (Washington, D.C.) 2023;6:0097

Cellular gp96 upregulates AFP expression by blocking NR5A2 SUMOylation and ubiquitination in hepatocellular carcinoma.

Qian L, Liang Z, Wang Z, Wang J, Li X, Zhao J, Li Z, Chen L, Liu Y, Ju Y, Li C, Meng S
Journal of molecular cell biology 2023 Nov 27;15(5)

Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling.

Chen X, Lv X, Gao L, Liu J, Wang W, Guo L, Frasinyuk MS, Zhang W, Watt DS, Liu C, Liu X
Cells 2023 Mar 31;12(7)

miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A.

Huang X, Jia Y, Shi H, Fan H, Sun L, Zhang H, Wang Y, Chen J, Han J, Wang M, Du J, Zhang J
Asian biomedicine : research, reviews and news 2023 Jun;17(3):124-135

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.

Luo Z, Xin D, Liao Y, Berry K, Ogurek S, Zhang F, Zhang L, Zhao C, Rao R, Dong X, Li H, Yu J, Lin Y, Huang G, Xu L, Xin M, Nishinakamura R, Yu J, Kool M, Pfister SM, Roussel MF, Zhou W, Weiss WA, Andreassen P, Lu QR
Nature communications 2023 Feb 10;14(1):762

The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.

Yang X, Wang ZP, Xiang S, Wang D, Zhao Y, Luo D, Qiu Y, Huang C, Guo J, Dai Y, Zhang SL, He Y
Journal of medicinal chemistry 2022 Jun 9;65(11):8040-8061

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Lei H, Xu HZ, Shan HZ, Liu M, Lu Y, Fang ZX, Jin J, Jing B, Xiao XH, Gao SM, Gao FH, Xia L, Yang L, Liu LG, Wang WW, Liu CX, Tong Y, Wu YZ, Zheng JK, Chen GQ, Zhou L, Wu YL
Nature communications 2021 Jan 4;12(1):51

LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.

Fan Y, Köberlin MS, Ratnayeke N, Liu C, Deshpande M, Gerhardt J, Meyer T
The Journal of cell biology 2021 Aug 2;220(8)

Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.

Cattrini C, Capaia M, Boccardo F, Barboro P
Cancer treatment and research communications 2020;25:100221

Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma.

Godel M, Morena D, Ananthanarayanan P, Buondonno I, Ferrero G, Hattinger CM, Di Nicolantonio F, Serra M, Taulli R, Cordero F, Riganti C, Kopecka J
International journal of molecular sciences 2020 Jun 24;21(12)

Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2α and miR-494.

Fang Y, Yang C, Zhang L, Wei L, Lin J, Zhao J, Peng J
BioMed research international 2019;2019:5953619

Identifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis.

Xiong Y, Yuan L, Chen L, Zhu Y, Zhang S, Liu X, Xiao Y, Wang X
Journal of Cancer 2018;9(21):3912-3922

3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

Li W, Liu J, Fu W, Zheng X, Ren L, Liu S, Wang J, Ji T, Du G
Journal of experimental & clinical cancer research : CR 2018 Jul 3;37(1):132

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Gao L, Xu Z, Wang Y, Sun B, Song Z, Yang B, Liu X, Lin Y, Peng J, Han G, Wang S, Tang Z
Oncology reports 2016 Feb;35(2):1101-8

Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.

Lu C, Liu X, Liu C, Wang J, Li C, Liu Q, Li Y, Li S, Sun S, Yan J, Shao J
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):588-606

High glucose promotes gastric cancer chemoresistance in vivo and in vitro.

Zhao W, Chen R, Zhao M, Li L, Fan L, Che XM
Molecular medicine reports 2015 Jul;12(1):843-50

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

Minajigi A, Froberg J, Wei C, Sunwoo H, Kesner B, Colognori D, Lessing D, Payer B, Boukhali M, Haas W, Lee JT
Science (New York, N.Y.) 2015 Jul 17;349(6245)

TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.

Li X, Liu Y, Chen W, Fang Y, Xu H, Zhu HH, Chu M, Li W, Zhuang G, Gao WQ
Oncotarget 2014 Oct 15;5(19):9498-513

Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: a proteomic study.

Liu JS, He SC, Zhang ZL, Chen R, Fan L, Qiu GL, Chang S, Li L, Che XM
Oncology reports 2014 Dec;32(6):2635-47

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 20233-1-AP(TOP2A-Specific antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human spleen using 20233-1-AP(TOP2A-Specific antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human spleen using 20233-1-AP(TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue