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- Antigen structure
- References [18]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 20233-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20233-1-AP, RRID:AB_10664923
- Product name
- TOP2A-Specific antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TOP2A-Specific antibody (Cat. #20233-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references TuBG1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways.
Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation.
Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling.
miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A.
Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.
The mechanism of acentrosomal spindle assembly in human oocytes.
Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.
LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.
Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.
Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma.
Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2α and miR-494.
Identifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis.
3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.
Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.
Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.
High glucose promotes gastric cancer chemoresistance in vivo and in vitro.
Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.
TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.
Zhang Y, Wang ZZ, Han AQ, Yang MY, Zhu LX, Pan FM, Wang Y
Hepatobiliary & pancreatic diseases international : HBPD INT 2024 Apr;23(2):195-209
Hepatobiliary & pancreatic diseases international : HBPD INT 2024 Apr;23(2):195-209
Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation.
Song C, Chen H, Yu B, Zhang L, Wang J, Feng C, Yang X, Tian X, Fan Y, Ji X, Wang H, Xie C, Zhang X
Research (Washington, D.C.) 2023;6:0097
Research (Washington, D.C.) 2023;6:0097
Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling.
Chen X, Lv X, Gao L, Liu J, Wang W, Guo L, Frasinyuk MS, Zhang W, Watt DS, Liu C, Liu X
Cells 2023 Mar 31;12(7)
Cells 2023 Mar 31;12(7)
miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A.
Huang X, Jia Y, Shi H, Fan H, Sun L, Zhang H, Wang Y, Chen J, Han J, Wang M, Du J, Zhang J
Asian biomedicine : research, reviews and news 2023 Jun;17(3):124-135
Asian biomedicine : research, reviews and news 2023 Jun;17(3):124-135
Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.
Luo Z, Xin D, Liao Y, Berry K, Ogurek S, Zhang F, Zhang L, Zhao C, Rao R, Dong X, Li H, Yu J, Lin Y, Huang G, Xu L, Xin M, Nishinakamura R, Yu J, Kool M, Pfister SM, Roussel MF, Zhou W, Weiss WA, Andreassen P, Lu QR
Nature communications 2023 Feb 10;14(1):762
Nature communications 2023 Feb 10;14(1):762
The mechanism of acentrosomal spindle assembly in human oocytes.
Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361
Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.
Yang X, Wang ZP, Xiang S, Wang D, Zhao Y, Luo D, Qiu Y, Huang C, Guo J, Dai Y, Zhang SL, He Y
Journal of medicinal chemistry 2022 Jun 9;65(11):8040-8061
Journal of medicinal chemistry 2022 Jun 9;65(11):8040-8061
LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.
Fan Y, Köberlin MS, Ratnayeke N, Liu C, Deshpande M, Gerhardt J, Meyer T
The Journal of cell biology 2021 Aug 2;220(8)
The Journal of cell biology 2021 Aug 2;220(8)
Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.
Cattrini C, Capaia M, Boccardo F, Barboro P
Cancer treatment and research communications 2020;25:100221
Cancer treatment and research communications 2020;25:100221
Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma.
Godel M, Morena D, Ananthanarayanan P, Buondonno I, Ferrero G, Hattinger CM, Di Nicolantonio F, Serra M, Taulli R, Cordero F, Riganti C, Kopecka J
International journal of molecular sciences 2020 Jun 24;21(12)
International journal of molecular sciences 2020 Jun 24;21(12)
Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2α and miR-494.
Fang Y, Yang C, Zhang L, Wei L, Lin J, Zhao J, Peng J
BioMed research international 2019;2019:5953619
BioMed research international 2019;2019:5953619
Identifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis.
Xiong Y, Yuan L, Chen L, Zhu Y, Zhang S, Liu X, Xiao Y, Wang X
Journal of Cancer 2018;9(21):3912-3922
Journal of Cancer 2018;9(21):3912-3922
3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.
Li W, Liu J, Fu W, Zheng X, Ren L, Liu S, Wang J, Ji T, Du G
Journal of experimental & clinical cancer research : CR 2018 Jul 3;37(1):132
Journal of experimental & clinical cancer research : CR 2018 Jul 3;37(1):132
Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.
Gao L, Xu Z, Wang Y, Sun B, Song Z, Yang B, Liu X, Lin Y, Peng J, Han G, Wang S, Tang Z
Oncology reports 2016 Feb;35(2):1101-8
Oncology reports 2016 Feb;35(2):1101-8
Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.
Lu C, Liu X, Liu C, Wang J, Li C, Liu Q, Li Y, Li S, Sun S, Yan J, Shao J
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):588-606
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):588-606
High glucose promotes gastric cancer chemoresistance in vivo and in vitro.
Zhao W, Chen R, Zhao M, Li L, Fan L, Che XM
Molecular medicine reports 2015 Jul;12(1):843-50
Molecular medicine reports 2015 Jul;12(1):843-50
Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.
Minajigi A, Froberg J, Wei C, Sunwoo H, Kesner B, Colognori D, Lessing D, Payer B, Boukhali M, Haas W, Lee JT
Science (New York, N.Y.) 2015 Jul 17;349(6245)
Science (New York, N.Y.) 2015 Jul 17;349(6245)
TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.
Li X, Liu Y, Chen W, Fang Y, Xu H, Zhu HH, Chu M, Li W, Zhuang G, Gao WQ
Oncotarget 2014 Oct 15;5(19):9498-513
Oncotarget 2014 Oct 15;5(19):9498-513
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20233-1-AP(TOP2A-Specific antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human spleen using 20233-1-AP(TOP2A-Specific antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human spleen using 20233-1-AP(TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
