Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-37757 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-TOP2A (Thr1343) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is HepG2 cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.
Vaughan CA, Singh S, Subler MA, Windle JJ, Inoue K, Fry EA, Pillappa R, Grossman SR, Windle B, Andrew Yeudall W, Deb SP, Deb S
Nature communications 2021 Jan 29;12(1):704
Nature communications 2021 Jan 29;12(1):704
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HepG2 cells treated with Ca2+ using TOP2A (pThr1343) polyclonal antibody (Product # PA5-37757) (left) or the same antibody preincubated with a blocking peptide (right).