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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- TA802537 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA802537, RRID:AB_2626386
- Product name
- TOP2A mouse monoclonal antibody,clone OTI3E11
- Antibody type
- Monoclonal
- Description
- TOP2A mouse monoclonal antibody,clone OTI3E11
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TOP2A
- Isotype
- IgG
- Antibody clone number
- OTI3E11
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA pH8.0)(1:600)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human Esophagus using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA pH8.0)(1:600)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA802537)(1:600)
- Validation comment
- IHC
