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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- V3443 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Topoisomerase II alpha Antibody / TOP2A
- Antibody type
- Monoclonal
- Description
- This highly specific Topoisomerase II alpha antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- TOP2A/1361
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human bladder carcinoma with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
