Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7577-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Topo II alpha Antibody
- Antibody type
- Monoclonal
- Description
- Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant human
Topo II alpha protein (amino acids
1326-1512) was used as the immunoge
n for this antibody.- Isotype
- IgG
- Antibody clone number
- ABM48B4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Expression analysis of Topo II alpha. Anti-Topo II alpha antibody (Clone: ABM48B4) was tested at 1 µg/ml on (1) HT-29, (2) 293, (3) A549 and (4) Raji Lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular Flow analysis of Topo II alpha antibody in Jurkat cells using 0.5 µg/ 10^6 cells of anti-Topo II alpha antibody (ABM48B4). Green represents isotype control;red represents anti-Topo II alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol