RQ5364

antibody from NSJ Bioreagents

Targeting: TOP2A TOP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5364

Provider

NSJ Bioreagents

Product name

TOP2A Antibody / Topoisomerase II alpha

Antibody type

Monoclonal

Description

This highly specific TOP2A antibody is suitable for use in Immunohistochemistry/Western blot applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

FB-20

Vial size

100 ul

Concentration

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Storage

Store the TOP2A antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa and 2) Jurkat cell lysate with TOP2A antibody. Predicted molecular weight ~174 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa and 2) Jurkat cell lysate with TOP2A antibody. Predicted molecular weight ~174 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon cancer with TOP2A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.