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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-81087 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TOP2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human TOP2A.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TOP2A in Lane A: HepG2 Whole Cell Lysate (30 µg), Lane B: Jurkat Whole Cell Lysate (30 µg) . Samples were probed using a TOP2A Polyclonal Antibody (Product # PA5-81087) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:174 kDa. Observed band size:174 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human TOP2A in human tonsil with TOP2A Polyclonal Antibody (Product # PA5-81087) (1:10000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human TOP2A in human ovarian cancer with TOP2A Polyclonal Antibody (Product # PA5-81087) (1:10000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human TOP2A in human lung cancer with TOP2A Polyclonal Antibody (Product # PA5-81087) (1:10000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of TOP2A in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with TOP2A Polyclonal Antibody (Product # PA5-81087) (1:5000) at 4° C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.
