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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- APRab19129 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- Topo IIα Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purification
- Reactivity
- Human, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Polyclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from HT-29 cells, using TOP2A Antibody. The lane on the right is blocked with the synthesized peptide.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of various cells using Topo IIα Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of 293 cells using Topo IIα Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of A549 cells, using TOP2A Antibody. The picture on the right is blocked with the synthesized peptide.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TOP2A Antibody. The picture on the right is blocked with the synthesized peptide.
