Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN955290 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1174-1204), (C-Term) antibody
- Antibody type
- Polyclonal
- Description
- Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 1174-1204, C-Term
- Vial size
- 0.4 mL
- Storage
- Store at 2 - 8°C for up to six months or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Mitotic chromosomes are constrained by topoisomerase II-sensitive DNA entanglements.
Kawamura R, Pope LH, Christensen MO, Sun M, Terekhova K, Boege F, Mielke C, Andersen AH, Marko JF
The Journal of cell biology 2010 Mar 8;188(5):653-63
The Journal of cell biology 2010 Mar 8;188(5):653-63
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (AP54331PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC