RQ6829

antibody from NSJ Bioreagents

Targeting: C5 C5a, C5b, CPAMD4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6829

Provider

NSJ Bioreagents

Product name

C5B Antibody / Complement C5B

Antibody type

Polyclonal

Description

This highly specific C5B antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the C5B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat liver, 2) rat liver, 3) rat RH35, 4) mouse liver, 5) mouse liver, 6) mouse RAW264.7 and 8) mouse ANA-1 cell lysate with C5B antibody. Predicted molecular weight ~189 kDa (C5), 75-80 kDa (beta chain).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse ANA-1 cells with C5B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C5B antibody.