16112-1-AP

antibody from Invitrogen Antibodies

Targeting: LPCAT1 AGPAT10, AGPAT9, AYTL2, FLJ12443

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16112-1-AP

Provider

Invitrogen Antibodies

Product name

LPCAT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IKRRAQSNG KWPQIMIFPE GTCTNRTCLI TFKPGAFIPG APVQPVVLRY PNKLDTITWT WQGPGALEIL WLTLCQFHNQ VEIEFLPVYS PSEEEKRNPA LYASNVRRVM AEALGVSVTD YTFEDCQLAL AEGQLRLPAD TCLLEFARLV RGLGLKPEKL EKDLDRYSER ARMKGGEKIG IAEFAASLEV PVSDLLEDMF SLFDESGSGE VDLRECVVAL SVVCRPARTL DTIQLAFKMY GAQEDGSVGE GDLSCILKTA LGVAELTVTD LFRAIDQEEK GKITFADFHR FAEMYPAFAE EYLYPDQTHF ESCAETSPAP IPNGFCADFS PENSDAGRKP VRKKLD (51-395 aa encoded by BC020166)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human lung tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat lung tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using (Product # 16112-1-AP) (LPCAT1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using LPCAT1 antibody 16112-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer slide using 16112-1-AP (LPCAT1 Antibody) at dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer slide using 16112-1-AP (LPCAT1 Antibody) at dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 16112-1-AP (LPCAT1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 16112-1-AP (LPCAT1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-LPCAT1 (IP:16112-1-AP, 3ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 3600ug.