PA5-26318

antibody from Invitrogen Antibodies

Targeting: LPCAT1 AGPAT10, AGPAT9, AYTL2, FLJ12443

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26318

Provider

Invitrogen Antibodies

Product name

LPCAT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Role of cardiolipins, mitochondria, and autophagy in the differentiation process activated by all-trans retinoic acid in acute promyelocytic leukemia.

Gianni' M, Goracci L, Schlaefli A, Di Veroli A, Kurosaki M, Guarrera L, Bolis M, Foglia M, Lupi M, Tschan MP, Cruciani G, Terao M, Garattini E
Cell death & disease 2022 Jan 10;13(1):30

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HeLa cells using a PCAT1 polyclonal antibody (Product # PA5-26318) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 ATRA effects on the expression of the genes belonging to the glycerophospholipid metabolism pathway in parental NB4, NB4-shCTRL, and NB4-shATG5 cells. A The effects exerted by ATRA on the genes belonging to the glycerophospholipid pathway in parental NB4 , NB4-shCTRL , and NB4-shATG5 cells were evaluated using the RNA-seq data generated from the three cell lines exposed to vehicle (DMSO) or ATRA (1 muM) for 48 h. The diagrams show the perturbations induced by ATRA on the expression of the indicated genes. The results presented in each box are expressed using the Log 2 of the ATRA-dependent fold-change values which we determined. The names of the genes whose expression is significantly increased by ATRA in the indicated cell-lines are written in red. The names of the genes whose expression is significantly decreased by ATRA in the indicated cell-lines are written in blue. When the indicated genes are significantly up-regulated (FDR > 0.05), the positive fold-change values are contained in a red box and are marked with an asterisk. When the indicated genes are significantly down-regulated (FDR > 0.05), the negative fold-change values are contained in a light blue box. B The column diagrams indicate the expression levels of the CRLS1 , LPCAT1 , and PGS1 mRNAs which were determined in three independent cultures of NB4-shCTRL and NB4-shATG5 cells, by PCR using specific Taqman Assays. The results are expressed as the Mean + S.D. ( N = 3) of the values determined. **Significa