H00004149-M01

antibody from Abnova Corporation

Targeting: MAX bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004149-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004149-M01, RRID:AB_425539

Product name

MAX monoclonal antibody (M01), clone 4E10-1A9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MAX.

Antigen sequence

MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFH
SLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNH
THQQDIDDLKRQNALLEQQVRALEKARSSAQLQTN
YPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEP
QSRKKLRMEAS


Isotype

IgG

Antibody clone number

4E10-1A9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAX is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to MAX on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAPK14 and MAX. HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)