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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- PA5-29745 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS.
- Concentration
- 0.83 mg/mL
Submitted references The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2.
Bianchi N, Brugnoli F, Grassilli S, Bourgeois K, Keillor JW, Bergamini CM, Aguiari G, Volinia S, Bertagnolo V
Cells 2021 Nov 6;10(11)
Cells 2021 Nov 6;10(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAX using 30 µg of HeLa nucleus lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MAX polyclonal antibody (Product # PA5-29745) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAX was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAX Polyclonal Antibody (Product # PA5-29745) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MAX Polyclonal Antibody (Product # PA5-29745). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX Polyclonal Antibody (Product # PA5-29745) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAX was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAX Polyclonal Antibody (Product # PA5-29745) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAX Polyclonal Antibody immunoprecipitates MAX protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of MAX protein by 2 µg of MAX Polyclonal Antibody (Product # PA5-29745) 15% SDS-PAGE The immunoprecipitated MAX protein was detected by MAX Polyclonal Antibody (Product # PA5-29745) diluted at 1:1,000.
