Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF5849 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal IL-21 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects canine IL-21 in direct ELISAs. In direct ELISAs, approximately 35% cross-reactivity with recombinant human IL-21 is observed, and less than 3% cross-reactivity with recombinant mouse IL-21 is observed.
- Reactivity
- Canine
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-21 and Neutralization by Canine IL-21 Antibody. Recombinant Canine IL-21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND50 is typically 2-10 µg/mL.